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Zoliclones are monoclinal antibodies that are obtained from the blood of laboratory mice.
Everything is done through genetic engineering. The blood group, in our time, the ABO system, is established precisely with the help of these antibodies. Extremely large and high activity and avidity are demonstrated by coliclones, which suggests the time of arrival and the severity of the agglutination (gluing) reaction.
This technique is used only in a full-fledged laboratory. Moreover, inside the cabinet, the temperature must be within the range starting from +15.0 and up to + 25.0 degrees Celsius. And the process of establishing a blood group can only occur with excellent lighting.

A Few Tips
— To avoid a decrease in antibody activity due to drying, reagents should only be stored tightly closed.
— It is not recommended to use reagents that contain flakes and are cloudy.
- To perform this work, you will need a special separate pipette, separately for each analysis.
- The determination process should be carried out on a special tablet or plate, usually white color is used, with a well-moistened surface. Since coliclones are characterized by sufficiently high avidity and activity, under such conditions, it is possible to use 1 series of anti-A reagents, as well as anti-B reagents.

Algorithm of actions
1. On the tablet should be marked: anti-A and anti-B.
2. Next, tsoliklones of approximately 0.10 ml each are dripped under the corresponding signatures.
It should be noted that the anti-A reagent has a yellowish-pink color, the anti-B reagent is blue. This should be remembered. 3. Then, near the monoclonal antibodies, it is necessary to drop a small drop of blood and mix it well with the reagents.
This should be done with a special glass rod or a corner of glass.
Moreover, if you have to mix the next couple of drops, then before this action, the stick or glass is well washed and wiped. 4. Over the next 2.5 minutes, agglutination is monitored. In the process of this, the tablet should be shaken a little. The next step is to evaluate the result.

Analysis of the result
1. If there is no agglutination with two colicons, then you have 1st blood;
2. If there is agglutination with anti-A tsoliklon, you have the 2nd group in front of you;
3. Agglutination with anti-B zoliclon indicates the 3rd blood group;
4. The presence of agglutination with a pair of coliclones is group 4, while it must be confirmed by agglutination with anti-AB coliclone in the absence of nonspecific agglutination relative to isotonic sodium chloride solution.

For clarity, we suggest you watch this video: